Isolation, characterization, and distribution of a novel neuropeptide, Rana RFamide (R‐RFa), in the brain of the European green frog Rana esculenta
Identifieur interne : 001343 ( Main/Exploration ); précédent : 001342; suivant : 001344Isolation, characterization, and distribution of a novel neuropeptide, Rana RFamide (R‐RFa), in the brain of the European green frog Rana esculenta
Auteurs : Nicolas Chartrel [France] ; Cynthia Dujardin [France] ; Jérôme Leprince [France] ; Laurence Desrues [France] ; Marie-Christine Tonon [France] ; Eric Cellier [France] ; Pascal Cosette [France] ; Thierry Jouenne [France] ; Guy Simonnet [France] ; Hubert Vaudry [France]Source :
- Journal of Comparative Neurology [ 0021-9967 ] ; 2002-06-24.
English descriptors
- KwdEn :
Abstract
A novel neuropeptide of the RFamide peptide family was isolated in pure form from a frog (Rana esculenta) brain extract by using reversed‐phase high performance liquid chromatography in combination with a radioimmunoassay for mammalian neuropeptide FF (NPFF). The primary structure of the peptide was established as Ser‐Leu‐Lys‐ Pro‐Ala‐Ala‐Asn‐Leu‐Pro‐Leu‐ Arg‐Phe‐NH2. The sequence of this neuropeptide, designated Rana RFamide (R‐RFa), exhibits substantial similarities with those of avian LPLRFamide, gonadotropin‐inhibitory hormone, and human RFRP‐1. The distribution of R‐RFa was investigated in the frog central nervous system by using an antiserum directed against bovine NPFF. In the brain, immunoreactive cell bodies were primarily located in the hypothalamus, i.e., the anterior preoptic area, the suprachiasmatic nucleus, and the dorsal and ventral hypothalamic nuclei. The most abundant population of R‐RFa–containing neurons was found in the periependymal region of the suprachiasmatic nucleus. R‐RFa– containing fibers were widely distributed throughout the brain from the olfactory bulb to the brainstem, and were particularly abundant in the external layer of the median eminence. In the spinal cord, scattered immunoreactive neurons were found in the gray matter. R‐RFa–positive processes were found in all regions of the spinal cord, but they were more abundant in the dorsal horn. This study provides the first characterization of a member of the RFamide peptide family in amphibians. The occurrence of this novel neuropeptide in the hypothalamus and median eminence and in the dorsal region of the spinal cord suggests that, in frog, R‐RFa may exert neuroendocrine activities and/or may be involved in the transmission of nociceptive stimuli. J. Comp. Neurol. 448:111–127, 2002. © 2002 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/cne.10253
Affiliations:
- France
- Aquitaine, Haute-Normandie, Nouvelle-Aquitaine, Région Normandie
- Bordeaux, Mont‐ Saint‐Aignan, Mont‐Saint‐Aignan
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Neurol.</title><idno type="ISSN">0021-9967</idno><idno type="eISSN">1096-9861</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>New York</pubPlace><date type="published" when="2002-06-24">2002-06-24</date><biblScope unit="volume">448</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="111">111</biblScope><biblScope unit="page" to="127">127</biblScope></imprint><idno type="ISSN">0021-9967</idno></series><idno type="istex">89A96DF1AB1C9B7410EF7707E23FF4CDA3DF0602</idno><idno type="DOI">10.1002/cne.10253</idno><idno type="ArticleID">CNE10253</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0021-9967</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>FMRFamide</term><term>amphibians</term><term>brain</term><term>neuropeptide FF</term><term>pituitary</term><term>spinal cord</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A novel neuropeptide of the RFamide peptide family was isolated in pure form from a frog (Rana esculenta) brain extract by using reversed‐phase high performance liquid chromatography in combination with a radioimmunoassay for mammalian neuropeptide FF (NPFF). The primary structure of the peptide was established as Ser‐Leu‐Lys‐ Pro‐Ala‐Ala‐Asn‐Leu‐Pro‐Leu‐ Arg‐Phe‐NH2. The sequence of this neuropeptide, designated Rana RFamide (R‐RFa), exhibits substantial similarities with those of avian LPLRFamide, gonadotropin‐inhibitory hormone, and human RFRP‐1. The distribution of R‐RFa was investigated in the frog central nervous system by using an antiserum directed against bovine NPFF. In the brain, immunoreactive cell bodies were primarily located in the hypothalamus, i.e., the anterior preoptic area, the suprachiasmatic nucleus, and the dorsal and ventral hypothalamic nuclei. The most abundant population of R‐RFa–containing neurons was found in the periependymal region of the suprachiasmatic nucleus. R‐RFa– containing fibers were widely distributed throughout the brain from the olfactory bulb to the brainstem, and were particularly abundant in the external layer of the median eminence. In the spinal cord, scattered immunoreactive neurons were found in the gray matter. R‐RFa–positive processes were found in all regions of the spinal cord, but they were more abundant in the dorsal horn. This study provides the first characterization of a member of the RFamide peptide family in amphibians. The occurrence of this novel neuropeptide in the hypothalamus and median eminence and in the dorsal region of the spinal cord suggests that, in frog, R‐RFa may exert neuroendocrine activities and/or may be involved in the transmission of nociceptive stimuli. J. Comp. Neurol. 448:111–127, 2002. © 2002 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Aquitaine</li><li>Haute-Normandie</li><li>Nouvelle-Aquitaine</li><li>Région Normandie</li></region><settlement><li>Bordeaux</li><li>Mont‐ Saint‐Aignan</li><li>Mont‐Saint‐Aignan</li></settlement></list><tree><country name="France"><region name="Région Normandie"><name sortKey="Chartrel, Nicolas" sort="Chartrel, Nicolas" uniqKey="Chartrel N" first="Nicolas" last="Chartrel">Nicolas Chartrel</name></region><name sortKey="Cellier, Eric" sort="Cellier, Eric" uniqKey="Cellier E" first="Eric" last="Cellier">Eric Cellier</name><name sortKey="Cosette, Pascal" sort="Cosette, Pascal" uniqKey="Cosette P" first="Pascal" last="Cosette">Pascal Cosette</name><name sortKey="Desrues, Laurence" sort="Desrues, Laurence" uniqKey="Desrues L" first="Laurence" last="Desrues">Laurence Desrues</name><name sortKey="Dujardin, Cynthia" sort="Dujardin, Cynthia" uniqKey="Dujardin C" first="Cynthia" last="Dujardin">Cynthia Dujardin</name><name sortKey="Jouenne, Thierry" sort="Jouenne, Thierry" uniqKey="Jouenne T" first="Thierry" last="Jouenne">Thierry Jouenne</name><name sortKey="Leprince, Jerome" sort="Leprince, Jerome" uniqKey="Leprince J" first="Jérôme" last="Leprince">Jérôme Leprince</name><name sortKey="Simonnet, Guy" sort="Simonnet, Guy" uniqKey="Simonnet G" first="Guy" last="Simonnet">Guy Simonnet</name><name sortKey="Tonon, Marie Hristine" sort="Tonon, Marie Hristine" uniqKey="Tonon M" first="Marie-Christine" last="Tonon">Marie-Christine Tonon</name><name sortKey="Vaudry, Hubert" sort="Vaudry, Hubert" uniqKey="Vaudry H" first="Hubert" last="Vaudry">Hubert Vaudry</name><name sortKey="Vaudry, Hubert" sort="Vaudry, Hubert" uniqKey="Vaudry H" first="Hubert" last="Vaudry">Hubert Vaudry</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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